GK072 ScienCell 人類適應(yīng)性免疫反應(yīng)qPCR檢測試劑盒，Human Adaptive Immune Response qPCR Array Kit

ScienCell 研究實驗室（ScienCell）是一家生物技術(shù)公司，其使命是實驗和治療用細胞和細胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞，細胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實現(xiàn)細胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商，為客戶提供，正規(guī)進口，貨期穩(wěn)定，折扣好的服務(wù)標(biāo)準(zhǔn)。

GK072 ScienCell人類適應(yīng)性免疫反應(yīng)qPCR檢測試劑盒，Human Adaptive Immune Response qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌： ScienCell

產(chǎn)品貨號：GK072

產(chǎn)品名稱：GK072 ScienCell人類適應(yīng)性免疫反應(yīng)qPCR檢測試劑盒，Human Adaptive Immune Response qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK072 ScienCell人類適應(yīng)性免疫反應(yīng)qPCR檢測試劑盒，Human Adaptive Immune Response qPCR Array Kit， 產(chǎn)品說明：

ScienCell的GeneQuery™人類適應(yīng)性免疫反應(yīng)qPCR陣列（GQH-AIR）有助于對參與適應(yīng)性免疫或獲得性免疫的88個關(guān)鍵基因進行基因表達分析。 脊椎動物有兩個主要的免疫系統(tǒng)：先天性和適應(yīng)性。 雖然先天免疫系統(tǒng)通常負責(zé)宿主防御病原體，但適應(yīng)性免疫系統(tǒng)負責(zé)防御特定病原體，從而賦予免疫記憶。 如何根據(jù)其在適應(yīng)性免疫反應(yīng)中的功能對所包含的基因進行分組的簡要示例如下所示：

抗原呈遞：CANX、HLA-A、HLA-DPB1、PSMB8、TAP2

Th1標(biāo)志物：IL12RB1，CCR5，CD94，CXCR3。IL18R

Th2 標(biāo)志物：IL13、IL1R1、IL4、CCR8、CSF2

Th17 標(biāo)記： CCR6， IL17A， IL6， RORC， IRF4

Treg 標(biāo)記物：FOXP3、CD4、IL10、IL2RA、CTLA4

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可識別并有效擴增特定靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有說明，否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Adaptive Immune Response qPCR Array (GQH-AIR) facilitates gene expression profiling of 88 key genes involved in adaptive immunity or acquired immunity.  Vertebrates have two main immune systems: innate and adaptive.  While the innate immune system is responsible for host defense against pathogens in a general way, the adaptive immune system is responsible for defense against particular pathogens, thereby conferring immunological memory.  Brief examples of how included genes may be grouped according to their function in the adaptive immune response are shown below:

·         Antigen presentation: CANX, HLA-A, HLA-DPB1, PSMB8, TAP2

·         Th1 markers: IL12RB1, CCR5, CD94, CXCR3. IL18R

·         Th2 markers: IL13, IL1R1, IL4, CCR8, CSF2

·         Th17 markers: CCR6, IL17A, IL6, RORC, IRF4

·         Treg markers: FOXP3, CD4, IL10, IL2RA, CTLA4

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人類適應(yīng)性免疫應(yīng)答 qPCR 陣列板布局*（8 個粗體和斜體對照）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

ScienCell基因表達分析產(chǎn)品訂購信息：

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